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文献和实验V294.PART II,Chapter 2 Boyden法体外趋化试验
., and Park, M. (2000)Activation of Cdc42, Rac, PAK, and Rho-kinase in response to hepatocyte growth factor differentially regulates epithelial cell colony spreading and dissociation. Mol. Biol. Cell 11, 1709–1725. 8. Ren, X. D., Kiosses, W. B., Sieg, D
V294.PART II,Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
, J., Lauffenburger, D. A., Turner, T., and Wells, A. (2001) Tumor invasionas dysregulated cell motility. Semin. Cancer Biol. 11, 105–117. 5. Zigmond, S. H. and Hirsch, J. G. (1973) Leukocyte locomotion and chemotaxis.New methods for evaluation
Methods for the Detection of D-Amino-Acid Oxidase
kidney D-amino-acid oxidase (Sigma, St. Louis, MO or Boehringer Mannheim, Germany) was used as a control. Organs, tissues, or tissue culture cells were homogenized in distilled water and centrifuged at 550 x g for 5 min. The supernatant solutions
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