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文献和实验Beta-gal Assay Adapted from Current Protocols in Molecular Biology 1. Grow 5ml YEPD cultures to mid-log phase. 2. Centrifuge and resuspend cells in 5ml Z-buffer, then place on ice. 3. Measure OD600. 4. Use straight
Bacterial beta-Galactosidase Histochemisty Bible
Bacterial beta-Galactosidase Histochemisty Bible This is a collection of procedures and lore associated with staining mammalian tissue for bacterial beta-galactosidase. Unfortunately, I can't attribute much of the development work
Beta-gal staining of eukaryotic cells in vitro
(Modification of methods of Dr. Seong-Seng Tan and Promega's "Protocols and Applications Guide") Cells previously transfected with a lac Z construct can be stained for b-galactosidase activity. 1. Wash cells 2 - 3 x with PBS (2 ml
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