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文献和实验Sporulation and Dissection of Yeast a/alpha Diploids
Sporulation and Dissection of Yeast a/alpha Diploids Linda Hoskins Last Modified Tue, Nov 6, 2001 These conditions work well for the Hieter yeast strains 1. Isolate diploid colonies. Inoculate 2 ml YPD + Ade with a single
Detection and Measurement of Radioactivity
Radioactive Decay Isotopes of a given element have nuclei with the same number of protons but different numbers of neutrons. Some isotopes are stable, however radioisotopes are unstable and disintegrate, with the emission
Replication timing by density transfer
), arrest with 200 nM alpha factor (1-1.25 doubling times, until % unbudded cells stops increasing). NOTE: This alpha factor concentration is for bar1 strains. BAR1+ strains require 15x more alpha factor. 3. Filter, wash in Ycomp+alpha factor, resuspend
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