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文献和实验Cell Culture Materials 1. Falcon Primaria culture dishes. 2. Culture medium: DME (or F12K) with glutamine, supplemented with 7% heat-deactivated horse serum (56oC for 30 min), 7% fetal calf serum, 50 units penicillin, 50 ug/ml
Transgenic Mouse and Transgenic Rat Outline
an endogenous mouse gene, such as beta-globin, or an endogenous rat gene, such as prolactin, is required in order to demonstrate that the DNA preparations are free of PCR inhibitors. Animals are tested with both assays so that no transgenic founder is mistakenly
Recovering DNA from agarose gels
A very popular method is to bind the DNA to silica particles by using commercially available binding resins, diatomaceous earth or glass fibers. This method was previously described in Methods and reagents [see TIBS 19,182-183]. Briefly, fragments from the gel
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