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文献和实验Isolation of Microtubules (Bovine Brain)
LEVEL II Materials Freshly removed bovine brain 2 Wire sieve (tea strainer) Microtubule buffer (MT buffer) 0.1 M MES (2-(N-Morphilino)ethanesulfonic acid) 1 mM EGTA (Ethylene Glycol-bis
Culture of BEND Cells (Bovine Endometrial Cells)
CULTURE MEDIUM Ingredients these catalog numbers refer to products from Sigma ; products from other suppliers can also be used Powdered Hams F-12 (N6760) Powdered MEM D-valine modification (M7395) Fetal bovine serum (F4135) Horse
TUNEL PROCEDURE IN BOVINE EMBRYOS
the sample with coverslip. 14) Determine the number of fluorescent nuclei. Red fluorescence indicates nonapoptotic cells and green/yellow (PI) or (Hoescht)fluorescence indicates apoptotic cells. Confocal image of a bovine
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