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货号：556296
名称：BLANK CALIBRATION PRTCLS 6.0-6.4 U M 5ML
品牌：BD biosciences
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文献和实验the top of the tube holder. The blank for this exercise is the solution containing no dopachrome, but all other chemicals. The amount of solution placed in the cuvette is not important, but is usually about 5 ml. It should approximately reach the bottom of the logo
(STANDARD ASSAY, 20-150 µ g protein; 200-1500 µ g/ml) Prepare a series of protein standards using BSA diluted with 0.15 M NaCl to final concentrations of 0 (blank = NaCl only), 250, 500, 750 and 1500 µ g BSA/ml. Also prepare serial dilutions
lipase substrate in 1.5 ml of 0.1 M Tris–HCl buffer at pH 8.0. Mixture was incubated for 6 h at 37 jC, after which hydrolysis was stopped by addition of 3 ml of 95% ethyl alcohol. The mixture was then titrated with 0.01 N NaOH using 0.9% (w/v
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