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货号：554781
名称：1X PBS WASH BUF 3X125ML
品牌：BD biosciences
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文献和实验band and serum with suction. (See attached figure, in preparation)Wash in PBS (or media A) to remove Histopaque (spin 1800 rpm/5 min at 4o C)Remove PBS (or media A) , add 18 ml cold H2 O to lyse RBC, and add 2 ml 10x PBS.Spin 1400 rpm/5min at 4o C.Wash
A general protocol for staining cell for cytometry analysis
Staining 1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of ~1 x 10 6 cells/ml. 2. Transfer 100 µl of the solution (~1 x 10 5 cells) to a 5 ml culture
In Situ Hybridization Using Digoxigenin Labeled Probes
dilution of Anti Digoxigenin-POD (Boehringer Cat. #1207 733). 1) Wash the slides in 1X PBS 2 times for 5 minutes each at room temperature 2) Wash for 2 minutes in 1X PBS/0.1 % Triton-X-100. 3) Rinse in 1X PBS 4) Place the slides horizontally
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