- ￥4672
- abcam
- ab35654
- 2026年04月23日
- ICC/IF, ELISA
- Mouse monoclonal [2D7/1] to E. coli LPS
- Escherichia coli
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
低温
- 是否单克隆：
单克隆
- 适应物种：
Escherichia coli
- 目录编号：
ab35654
- 宿主：
Mouse monoclonal [2D7/1] to E. coli LPS
- 应用范围：
ICC/IF, ELISA
- 浓度：
500ug/ml
- 抗原：
ab35654
- 抗体英文名：
Anti-E. coli LPS
- 抗体名：
Anti-E. coli LPS
- 规格：
100ug
Key features and details
- Mouse monoclonal [2D7/1] to E. coli LPS
- Suitable for: ICC/IF, ELISA
- Reacts with: Escherichia coli
- Isotype: IgG2b
-
产品名称
Anti-E. coli LPS抗体[2D7/1] -
描述
小鼠单克隆抗体[2D7/1] to E. coli LPS -
宿主
Mouse -
特异性
The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The J5 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).
The clone 2D7/1 has also been found to react with K. pneumoniae, S. sonnei and S. typhimurium.
-
经测试应用
适用于: ICC/IF, ELISAmore details -
种属反应性
与反应: Escherichia coli -
免疫原
Tissue, cells or virus corresponding to Escherichia coli E. coli LPS. Escherichia coli J5 cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
存储溶液
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS浓度- 100 µg 浓度为 0.5 mg/ml
应用 Ab评论 说明 ICC/IF (3) 1/200.ELISA (1) 1/1500.
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- 作者
- 内容
- 询问日期
文献和实验Transformation of E. coli by Electroporation
Materials: SOB medium E. coli host strain such as DH5α WB tRNA 5 M ammonium acetate
In recent years several techniques have been described for the introduction of DNA molecules into Escherichia coli . These are based on the findings of Mandel and Higa (1 ), who demonstrated that incubation of cells with naked bacteriophage DNA
1.O/N (5ml E.coli in LB-->1ml into 200ml LB/37℃ 2.Grow 2-3H to A660 ~0.3-0.4 3.Chill cells 10'/ice Spin down 10',5K,4℃ 4.Wash 1X 200ml 10% glycerol (sterile)40℃; Spin down 10',5K,4℃ 5.Wash 1X 40 ml 10% glycerol--> transfer to S34 tubes & spin
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