Trevigen offers a number of streptavidin conjugates to allow flexibility in both in situ labeling, and our flow cytometric-based assays. Strep-HRP is a versatile conjugate as demonstrated by its inclusion in all Trevigen’s colorimetric TACS®, TACS ·XL® and DNA damage detections assays.
Streptavidin, MW 60,000, is covalently linked to horseradish peroxidase by a modification of the periodate method with a molar enzyme/protein ratio of 2.5:1. The conjugate binds four molecules of biotin with high affinity (K d = 10-15 M-1). The conjugate is provided in liquid form with a proprietary stabilizer added, without preservative.
Source: Streptavidin isolated from Streptomyces avidinii
Recommended starting dilutions: TACS ® In Situ applications: 1:50 (for DAB based kits) to 1:750 (for TACS Blue Label™ based kits).