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- 英文名：
50% Glycerol, purified
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1
- 供应商：
甄准生物
- 规格：
250ML
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文献和实验High Throughput Screening of Purified Proteins for Enzymatic Activity
Understanding the functions of every protein in the proteome is one of the great challenges of the postgenomic era. Global genome sequencing efforts revealed that in any genome 30–50% of genes encode proteins with unknown function
Purification of Gcn4 James Fishburn,Hahn Lab March 2006 Gcn4 constructs are cloned into the pET21(a)vector and have a 6-His tag expressed at the N-terminus Gcn4 is expressed and purified from BL21(DE3)RIL cells Day 1 Grow 10+ ml culture
required to kill 50% of J774A.1 cells (50% effective concentration [EC50]) when they were incubated with PA (1 μg/ml) at 37°C (Table 1). The protein was measured by the method described by Lowry et al.. By using this procedure, rLF was purified
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