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- 2025年07月13日
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- 文献和实验
- 技术资料
- 英文名：
Fe (Iron) 1000ppm in mineral oil
- 库存：
1
- 供应商：
甄准生物
- 规格：
100G
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文献和实验centrifuge to collect all drops from walls of tube. Overlay the sample with half volume of mineral oil or add an appropriate amount of wax. This step may be omitted if the thermal cycler is equipped with a heated lid. Place samples in a thermocycler
. See Enhancers & Inhibitors in this Guide.· Enhancers needed. Some reactions may amplify only in the presence of additives. See Enhancers & Inhibitors in thisGuide.· Mineral oil problem. The reaction must be overlaid with highquality, nuclease-free light mineral
The ribonuclease protection assay (RPA)
12. Dilute the probe from Step 8 with hybridization buffer to the appropriate concentration: Add 2 µl of diluted probe to each RNA sample and mix by pipetting. Add a drop of mineral oil to each tube and quick spin in the microfuge. 13
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