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文献和实验T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
Construction and Manipulation of Large-Insert Bacterial Clone Libraries
Introduction Construction of Large-Insert Bacterial Clone Libraries Fig. 1. A general procedure
-human CD3: Clone OKT3 (Functional Grade Cat. No. 16-0037) or Clone HIT3a (Functional Grade Cat. No. 16-0039) Complete RPMI-1640 Sterile PBMC 96-well flat-bottom microtiter plates with lids (Costar Cat. No. 3596) MTT Buffer MTT
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