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文献和实验, F., Craik, C.S., and Ellman, J.A. 2000. Synthesis of positional‐scanning libraries of fluorogenic peptide substrates to define the extended substrate specificity of plasmin and thrombin. Nat. Biotechnol. 18:187‐193
High-Throughput Screening of Peptide Deformylase Inhibitors
. In the third method, f-Met-Lys-7-amino-4-methylcoumarin is used as the substrate. Deformylation by PDF gives an excellent substrate for dipeptidyl peptidase I, which releases the dipeptide Met-Lys and fluorogenic 7-amino-4-methylcoumarin. The combination
Peptide Inhibition ELISA Protocol
Dilute specific and non-specific peptides to 200 mg/ml in PBT. In a microtiter plate, mix equal volumes of the peptide dilutions and the MAb supe. Add an equal volume of PBT and MAb as the control. Incubate 1 hour at 37
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