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文献和实验-nitrophenyl phosphate as substrate. Bone Gla protein(BGP) was measured by radioimmunoassay.Type I collagen,MMP-1,TIMP-1 mRNA was examined using semiquantitive reverse transcription-polymerase chain reaction(RT-PCR) analysis.Human osteosarcoma cell lines MG-63
, and bronchoalveolar lavage, in which a multitude of hydrolases act in concert. This unit describes a protocol to map the global proteolytic substrate specificities of complex biological samples using a concise combinatorial library of internally quenched fluorogenic
A Method for Assaying Deubiquitinating Enzymes
using 125 I-labeled Ub-PESTc. We also compare the sensitivity of this method to that using a fluorogenic peptide substrate, carbobenzoxy-LRGG-7-amido-4-methylcoumarin (Cbz-LRGG-AMC), that has also been used as a substrate for DUBs (19 ). 20
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