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文献和实验Western Blotting with Biotinylated Antibodies
ELECTROPHORESIS Guidelines for choosing the percent gel to be used for certain molecular weight proteins (based on 37:1 acralyamide: bis acraylamide ratio) 4-5% gels: > 250 kDa 7.5% gels: 250-120 kDa 10% gels: 120-40 kDa 13% gels: 40-15 kDa 15% gels: Gel
Western Blotting with Biotinylated Antibodies
buffer (1% BSA, 10mM Tris pH 7.5, 100mM NaCl, 0.1% Tween 20). Incubate the blot for 30 minutes at 37°C, 1 hour at room temperature, or overnight at 4°C. Incubation with Biotinylated Primary Antibody Primary antibody Dilute
Procedures for In Vitro Production of Bovine Embryos
Procedures for In Vitro Production of Bovine EmbryosThe procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC)] of embryos described here are based on procedures developed
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