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文献和实验T-Cell Activation Using mAb to CD3
of spleen cells gives a good response. If experimental conditions require, a titration of cell densities (2-3x106 /ml to 105 /ml) should be performed for optimization. After washing the wells with PBS (step 6 above), add 200µl of the cell suspension
Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
complexes from human HeLa S3 cells in a scale and purity optimized for characterization by mass spectrometry. For this purpose, we use stably expressed One-STrEP -tag ® fusion proteins. This approach was successfully used in characterization of histone
Purification of Human Multiprotein Complexes using OneSTrEP Technology
Introduction Here we describe a strategy for isolation of multiprotein complexes from human HeLa S3 cells in a scale and purity optimized for characterization by mass spectrometry. For this purpose, we use stably expressed
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