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文献和实验Protein A Mimetic (PAM) Affinity Chromatography: Immunoglobulins Purification
Antibodies of the G class can be conveniently purified, even at large scale, by affinity chromatography using immobilized protein A or G. Because specific and cost-effective ligands are not available, scaling up purification of immunoglobin
Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies
Repertoires of antibody (Ab) V genes derived from nonimmunized human donors (1 ) or made synthetically (2 3 ) have been cloned for display on filamentous bacteriophage as either scFvs or Fabs fused to the minor phage coat protein (pIII
Introduction of human 14-3-3 proteins
of the tryptic peptides derived from the purified enzyme revealed two amino acid sequences homologous to the 14-3-3 proteins.A polyclonal antibody was raised against purified ST-IV.A 33 kDa protein was co-immunoprecipitated from rat brain extracts with the anti
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