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文献和实验Polyclonal and Monoclonal Antibodies
are immunized essentially as described above. Injections: 1. First in Freund's complete 100-200ug/1ml injection, two sites (over each shoulder). 2. Second 1 month later in Freund's INCOMPLETE as above. 3. Bleed 2 weeks later. Boost 2 weeks
interaction network.This has facilitated discovery of novel pluripotency factors and a better understanding of stem cell pluripotency. Here we describe detailed procedures for an in vivo biotinylation system setup in mouse ES cells, and affinity purification
Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies
) (4 ). Phage Ab repertoires can be subjected to multiple rounds of panning on individual immobilized antigens (Ags) in order to isolate individual Ag-binding clones. This approach has been successfully applied to numerous purified soluble molecules, yielding
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