| 溶液成分 |
不同体积(ml)凝胶液中各成分所需体积(ml) |
| 5 |
10 |
15 |
20 |
25 |
30 |
40 |
50 |
| 6% |
|
|
|
|
|
|
|
|
| 水 |
2.6 |
5.3 |
7.9 |
10.6 |
13.2 |
15.9 |
21.2 |
26.5 |
| 30%
溶液 |
1.0 |
2.0 |
3 |
4 |
5 |
6 |
8 |
10 |
| 1.5 mol/L Tris (pH8.8) |
1.3 |
2.5 |
3.8 |
5 |
6.3 |
7.5 |
10 |
12.5 |
| 10% SDS |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
| 10%过硫酸氨 |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
| TEMED |
0.004 |
0.008 |
0.012 |
0.016 |
0.02 |
0.024 |
0.032 |
0.04 |
| 8% |
|
|
|
|
|
|
|
|
| 水 |
2.3 |
4.6 |
6.9 |
9.3 |
11.5 |
13.9 |
18.5 |
23.2 |
| 30%
溶液 |
1.3 |
2.7 |
4 |
5.3 |
6.7 |
8 |
10.7 |
13.3 |
| 1.5 mol/L Tris (pH8.8) |
1.3 |
2.5 |
3.8 |
5 |
6.3 |
7.5 |
10 |
12.5 |
| 10% SDS |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
| 10%过硫酸氨 |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
| TEMED |
0.003 |
0.006 |
0.009 |
0.012 |
0.015 |
0.018 |
0.024 |
0.03 |
| 10% |
|
|
|
|
|
|
|
|
| 水 |
1.9 |
4 |
5.9 |
7.9 |
9.9 |
11.9 |
15.9 |
19.8 |
| 30%
溶液 |
1.7 |
3.3 |
5 |
6.7 |
8.3 |
10 |
13.3 |
16.7 |
| 1.5 mol/L Tris (pH8.8) |
1.3 |
2.5 |
3.8 |
5 |
6.3 |
7.5 |
10 |
12.5 |
| 10% SDS |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
| 10%过硫酸氨 |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
| TEMED |
0.002 |
0.004 |
0.006 |
0.008 |
0.01 |
0.012 |
0.016 |
0.02 |
| 12% |
|
|
|
|
|
|
|
|
| 水 |
1.6 |
3.3 |
4.9 |
6.6 |
8.2 |
9.9 |
13.2 |
16.5 |
| 30%
溶液 |
2 |
4 |
6 |
8 |
10 |
12 |
16 |
20 |
| 1.5 mol/L Tris (pH8.8) |
1.3 |
2.5 |
3.8 |
5 |
6.3 |
7.5 |
10 |
12.5 |
| 10% SDS |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
| 10%过硫酸氨 |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
| TEMED |
0.002 |
0.004 |
0.006 |
0.008 |
0.01 |
0.012 |
0.016 |
0.02 |
| 15% |
|
|
|
|
|
|
|
|
| 水 |
1.1 |
2.3 |
3.4 |
4.6 |
5.7 |
6.9 |
9.2 |
11.5 |
| 30%
溶液 |
2.5 |
5 |
7.5 |
10 |
12.5 |
15 |
20 |
25 |
| 1.5 mol/L Tris (pH8.8) |
1.3 |
2.5 |
3.8 |
5 |
6.3 |
7.5 |
10 |
12.5 |
| 10% SDS |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
| 10%过硫酸氨 |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
| TEMED |
0.002 |
0.004 |
0.006 |
0.008 |
0.01 |
0.012 |
0.016 |
0.02 |
2.配制Tris-甘氨酸SDS-PAGE凝胶电泳5%积层胶(浓缩胶)所用溶液 上海甄准生物科技有限公司
| 溶液成分 |
不同体积(ml)凝胶液中各成分所需体积(ml) |
| 1 |
2 |
3 |
4 |
5 |
6 |
8 |
10 |
| |
|
|
|
|
|
|
|
|
| 水 |
0.68 |
1.4 |
2.1 |
2.7 |
3.4 |
4.1 |
5.5 |
6.8 |
| 30%
溶液 |
0.17 |
0.33 |
0.5 |
0.67 |
0.83 |
1 |
1.3 |
1.7 |
| 1.0 mol/L Tris (pH6.8) |
0.13 |
0.25 |
0.38 |
0.5 |
0.63 |
0.75 |
0 |
1.25 |
| 10% SDS |
0.01 |
0.02 |
0.03 |
0.04 |
0.05 |
0.06 |
0.08 |
0.1 |
| 10%过硫酸氨 |
0.01 |
0.02 |
0.03 |
0.04 |
0.05 |
0.06 |
0.08 |
0.1 |
| TEMED |
0.001 |
0.002 |
0.003 |
0.004 |
0.005 |
0.006 |
0.008 |
0.01 |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。