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文献和实验Optimal arbitrary primer length for Differential Display（图）
suggests that the optimal selectivity of the arbitrary primers should be provided by no more than 7 bases for each pair of primers to display 50-100 mRNAs.The minimum primer length for PCR is 9 bases.The arbitrary primers have to hybridize in a predictable
Primer Dimer problems in real-time PCR-Real-Time PCR
. However, most of primers designed by Primer Express seem to have many dimer formation by upper or lower primers themselves or Upper-lower dimers. So what I can do is only choosing the set with smallest no, of dimer. So I have performed the normal PCR again and run gel
1. Pull out eight glycerol stock plates from the �80oC freezer and set on bench top to thaw. Be sure to remove the foil seal before leaving the plates to thaw. 2. Master mix: Per 100ul RXN
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