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文献和实验E.Z.N.A.® Viral RNA Spin Protocol
body fluid or urine into the micro-centrifuge tube containing Lysis Buffer. Mix throughly by vortexing at maxi speed for 30 seconds. 3. Incubate at room temperature for 5-10 minutes. Briefly spin to collect any liquid from lid. 4. Add 560 ul
, and chromosomal DNA by pouring through a double-layer of cheesecloth into a new 50 ml conical tube. Centrifuge at 3,000 rpm for 20 minutes at 4degC in the Beckman GPR tabletop centrifuge. 6. Decant the supernatant to a 50 ml polypropylene centrifuge tube, add
Procedures for In Vitro Production of Bovine Embryos
50-ml centrifuge tubes. Be careful not to overfill the tube which can cause oocyte loss. A good measurement is the 45-ml line.Note: The number of tubes needed for the collection of oocytes depends upon the amount of medium you begin with in your 400
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