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文献和实验Blood collection and administration of fluids and drugs (mouse)
tubes Blood collection from the orbital sinus of mice is frequently used. One quarter ml can be repeatedly collected from mice at weekly intervals from alternate sides. Bleeding requires that the tube be directed into the orbital sinus (picture
by filtration into a collection tube. The collected DNA is concentrated by ethanol precipitation and crudely assayed for concentration and purity by agarose gel electrophoresis against known standards. The approximate yield of double-stranded DNA is 3-5 ug
Procedures for In Vitro Production of Bovine Embryos
hood 100 ml pipet tips and pipettor OMM + supplements Mineral oil 60 x 15 petri dishes Plastic pasteur pipet 3-4 bottles of OCM + supplement (warmed @ 38.5°C) 1 L saline + Pen/Strep (warmed @ 38.5°C)Preparation for Oocyte Collection Scalpel Petri dishes
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