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文献和实验A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
. Vortex the tube for 30 seconds. 18. Centrifuge at maximum speed ($13000 x g) at 4°C for 3 minutes. Aspirate the supernatant and air dry the RNA pellet by inverting the tube on a absorbent paper for 5-10 minutes 19. Add 50-100μl of DEPC
After lysing the samples. DNA or RNA needs to be purified. If it is RNA sample the lysis buffer must contain something (2-merceptoethanol) that will destroy all RNAse enzyme activity. Then you can purify samples in two ways, a) Phenol
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