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文献和实验A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
Fractionation of Nuclear and Cytoplasmic RNA by Trizol--分别
5 seconds again. Quick spin 30 seconds at maxi speed at RT. 6: Transfer cytoplasmic fraction to a new tube, put nuclear samples on ice now. 7: T℃ytoplasmic fractions, add 0.9ml 1.2xTRIzol reagent. Vortex to mix completely, sit at RT for 5 minutes
HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export
The requirement of HIV-1 and HTLV-1 to export incompletely spliced mRNAs has necessitated the evolution of Rev and Rex, respectively, to overcome host cellular mechanism that block nuclear-cytoplasmic export of incompletely processed mRNAs
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