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- 抗体英文名：
ANTIGENPLUS BUFFER SET
- 规格：
1SET
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文献和实验ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
& alternative recipe at end for lysis buffer and IP Dilution Buffer) 2. Add acid-washed glass beads, so there is a 1 to 2mm layer of liquid above the beads. 3. Vortex in the Tomy Mixer. Set it to level 10, mix 8 times for 1 minute each, and put on ice
Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
Steve HahnProtein Dilution Buffer 5ml20 mM Tris pH7.9 100 microliters 1M Tris 7.9150 mM KCl 0.75 ml 1 M KCl1 mM DTT 50 microliters 0.1 M DTT10% glycerol 1 ml 50% glycerol50 micrograms/ml BSA 2.5 microliters 100 mg/ml BSA3.1 ml H2OOptional: add Brij
no special instrumentation is necessary, and can be used to validate the quality of protein preparations, screen for ligands or cofactors, and discover buffers and additives that maximize protein stability. This unit describes how to set up a Thermofluor
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