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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
(DE3) COMPETENT CELL SET 2
- 供应商：
北京智杰方远科技
- CAS号：
******
- 保存条件：
详见说明
- 规格：
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文献和实验CCMB buffer to make chemical competent cell
TOP10 chemically competent cells ( modified from http://openwetware.org/wiki/TOP10_chemically_competent_cells#CCMB80_buffer) I also use this protocol to make Ecoli compent cell. It is pretty good. You can start from the fresh clone on plate
TOP10 chemically competent cells
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free for these protocols. The easiest way to do this is to avoid washing glassware, and simply rinse it out. Autoclaving glassware filled
kit. 2) Transform plasmid into competent HT115(DE3) bacterial cells and plate onto standard LB+ tetracycline+antibiotic plates. (Easy competent cell and transformation protocols below)note: the HT115 cells were a generous gift from D. Court (NCI
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