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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
APROTININ, BOV., RECOM, ANIMAL-FREE
- 供应商:
北京智杰方远科技
- CAS号:
******
- 保存条件:
详见说明
- 规格:
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文献和实验Isolation of cell nuclei for the application in the cell-free system
Leupeptin 100 µl of 1 mg/ml Pepstatin 100 µl of 1 mg/ml Aprotinin 37.65 ml H2O nuclease free NUCLEI STORAGE BUFFER (NSB): COMPOSITION: RECIPE for 1 ml: 10 mM PIPES (pH 7.4) 5 mM EGTA 80 mM KCl 20 mM NaCl 50% Glycerol 250 mM Sucrose
Preparation of cytoplasmic extracts for the application in a cell-free system
EGTA 250 µl of 1 M KCl 100 µl of 100 mM MgCl 5 µl of 1 M DTT 50 µl of 10 mM PMSF 10 µl of 1 mg/ml Leupeptin 10 µl of 1 mg/ml Pepstatin 10 µl of 1 mg/ml Aprotinin 2.04 ml H2O nuclease free
7.5,83 ml nuclease-free water •Protease inhibitors (200mM PMSF in isopropanol,2 mg/ml Aprotinin,2 mg/ml Leupeptin)•37% reagent grade Formaldehyde•1.25 M glycine•1% Agarose gel/EtBr1.Transfect,induce,or grow 1 to 5x106 cells in 10 cm dish.2.Rinse cells
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