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- 565765-1MG
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
g40-Secretase Inhibitor I
- 规格：
1MG
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
type of interest, grown under appropriate cell culture conditions with appropriate medium Cells, bacterial, competent, high-efficiency ( 5 x 109 cfu/µg DNA) XL10-Gold ultracompetent cells (Stratagene) have produced optimal results.
below is one example of differential gene expression analysis of cells obtained from microdissected tissue. 1. Materials Stratagene RAP-PCR kit 5X RT buffer (GenHunter) dNTP, 250 µM (GenHunter) dNTP, 25 µM (GenHunter) RNase inhibitor (Perkin
Eccles:Protein Lysates from Cells in Culture
Cell Lysis Buffer 5mL 0.1M Tris HCl pH 8 (10mM) 0.44g NaCl (150mM) 0.02g EDTA (1mM) 0.5mL nonidet P40 (1% w/v) 0.05g SDS (0.1% w/v) Make up to 50mL with MQH2 O and filter sterilise. Store at 4 degrees C. This cell lysis
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