Glycoprotein Deglycosylation K

Deglycosylation of Glycoproteins Using Endoglycosidases

  T.H. Plummer, Jr. and A.L. Tarentino, Department of Biochemistry, New York State Department of Health, Wadsworth Center for Laboratories and Research, Albany, New York 12201 Parameters of deglycosylation vary with every glycoprotein

O-Glycoprotein Biosynthesis: Site Localization by Edman Degradation and Site Prediction Based on Random Peptide Substrates

-glycosylation, significantly hinders our understanding of mucin-type O-glycosylation at almost every level from their biosynthesis to their biological and biophysical properties. In this chapter, the use of partial chemical deglycosylation coupled with Edman

Plant Proteomics and Glycosylation

and the oligosaccharide moiety. In this review, we present different methods, commonly used in our laboratory, to study the glycosylation of plant proteins. These approaches rely on blot detection with glycan-specific probes, as well as specific deglycosylation