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文献和实验Chromosomal DNA Extraction from Gram-positive Bacteria
This procedure was originally developed for Listeria monocytogenes but has worked well with other Gram+ bacteria we've tried. 1.Pellet cells from 10 ml overnight cultures in BHI or LB and wash in 5 ml of 0.1X SSC. 2.Resuspend in 1 ml 10 mM Tris
Antibody Production by the Gram-Positive Bacterium Bacillus megaterium
but they showed only a limited capacity to secrete antibody fragments into the medium – a prerequisite for easy downstream processing. Gram-positive bacteria are known to efficiently secrete recombinant proteins into the medium. Recently, we demonstrated
Molecular Investigation of Glycopeptide Resistance in Gram-Positive Bacteria
Our original intention was for this chapter to be written by Sandra Handwerger (Rockefeller University, New York), but tragically she died in April 1996 before she could undertake the task. In our opinion, Sandra was one of the leading research
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