Anti-Dinitrophenyl Hapten Mono

Isolation of Anti-Hapten Specific Antibody Fragments from Combinatorial Libraries

system unless presented as an epitope conjugated to a suitable immunogenic carrier protein, such as bovine thyroglobulin. The principal interest in anti-hapten Abs is as detection molecules for use in diagnostic assays. These typically use dipstick (qualitative

Monoclonal Antibody Production

the antigen you want the MAb directed against.If you are raising the MAbs against a small hapten that you coupled to a carrier protein for immunization of the mice,then use the hapten coupled to a different carrier protein for the screen. 4. Expanding

单克隆抗体 monoclonal antibody

　　指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定