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文献和实验E.Z.N.A.® HP Total RNA Kit Spin Protocol Eukaryotic Cells and Tissues
to the column. With the spin column inside a 2 ml collection tube (supplied with kit), centrifuge at 10,000 x g for 30-60 seconds min at room temperature. Discard flow-through and proceed to step 6. 6. Place column in a new 2 ml collection tube and add 300
the SDS and lyticase solutions, CHROMA SPIN-1000 DEPC-H2O Columns, and 2-ml centrifuge tubes for use with the columns.• Appropriate SD liquid or agar medium to keep selection on the plasmids (Appendix C.A;Appendix E).• Sterile, 1.5-ml microcentrifuge tubes
3. Remove DTT from Gcn4 using Zebra 0.5 ml Desalt Spin Column equilibrated and eluted with Gcn4 dilution buffer 4. Transfer volume of Gcn4 to be labeled to a 1.5 ml tube, freeze remaining Gcn4 and store at -70 deg 5. Keep Gcn4 for labeling
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