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文献和实验GPC Spin Column HPLCESI-MS Methods for Screening Drugs Noncovalently Bound to Proteins
Secondary drug screening methods are described for determining the relative degree of non-covalent binding between drug candidates and a protein of therapeutic interest by gel centrifugation chromatography using GPC spin columns for isolating
Fastfilter Plasmid Midi Kit Spin Protocol
into a 15 ml collection tube, provided. Add 2 ml of Buffer GPS to the column and Lit it sit at room temperature for 3-10 min. Spin in a swinging bucket rotor at 3,000-5,000 x g for 5 minutes at room temperature. Discard the eluate and assembled the column
• stabilizer soln (10% sodium thiosulfate+0.1N NaOH) • IODO-BEAD™(PIERCE) • Hamilton microsyringe (model 702, 25µl, needle gauge 22S, point style #2) • desalting column (Bio-rad DG-10 etc.) • column stand For procedure 2 • PBS
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