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文献和实验Easy Two-Step Method for Randomizing and Cloning Gene Fragments
and often hampered by lack of unique and convenient restriction sites. Here, we report an easy two-step method that produces a more balanced mutational spectrum and simplifies the cloning of randomly mutagenized genes or gene fragments for constructing high titer
water in a 1.5 ml microcentrifuge tube. The suspension will contain about 5 x 108 cells. Cells grown overnight in 2x YPAD broth will reach a titer between 1 and 2 x 108 /ml; the titer in SC medium will be about 5 x 107 /ml. Harvest 2 ml of a YPAD culture
Easy Way to Clone Genes From a Phage Library
Easy Way to Clone The protocol is oriented towards a C. albicans genomic library I made in Lambda Zap II on 7/97. The overall sequence of events is: • Titer and plate out phage • Lift plaques onto filters
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