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文献和实验RNA Purification Protocol for 0.1-2.5 mg of Animal Tissue
buffer. Cellular proteins and genomic DNA are removed by precipitation with PNP Buffer while RNA will remain in solution. RNA is further purified by isopropanol precipitation. 实验试剂 1. Isopropanol 2. 70% ethanol 实验
Purification of recombinant sBRF M166L
Renaturation of BRF protein Thaw Ni-NTA purified samples. Dilute the sample to a concentration of 0.25 to 0.5mg/ml in the elution buffer. To the BRF sample, add 10% Brij58 detergent to a final concentration of 0.1% Brij, add DTT to 5 mM final concentration
Purification of recombinant sBRF M166L
Tris 300mM NaCl 0.87 g NaCl 8.0M Urea 24 g Urea 100mM Imidazole 0.33 g Imidazole H2O to 50 ml finalRenaturation of BRF protein Thaw Ni-NTA purified samples.Dilute the sample to a concentration of 0.25 to 0.5mg/ml in the elution buffer
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