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文献和实验Due to their similarities in size and charge, complete resolution of histones by electrophoresis poses a considerable challenge. The addition of nonionic detergents to the traditional acetic acid/urea (AU) polyacrylamide gel electrophoresis (PAGE) system has afforded
2-D Polyacrylamide Gel Electrophoresis
: Use electrophoresis grade reagents to prepare the following solutions: A: 50 ml IEF Lysis Buffer 1.Add 21 g urea to 35 ml HPLC-grade H2O to a 50 ml Falcon tube (final concentration 7 M). 2.Vortex vigorously for several minutes. 3.Add: 7.6 g
二维-电泳疑难解答（Trouble shooting for 2D - Electrophoresis）
Appendix : Trouble shooting 1 General aspects • Quality of chemicals should be at least of analytical grade (p.a.) • Double-distilled or deionized (Millipore) water (conductivity
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