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文献和实验Clonality - X Chromosome Inactivation Assay
(1:1:1, v:v:v) (Gibco) 3 M Na Acetate, pH 5.2 (Life Technologies) Isopropanol Glycogen, 10 mg/ml (GenHunter) 70% ethanol Buffer 1 (50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 50 mM NaCl) Hha I restriction enzyme (Life Technologies) REact 2 Buffer
SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
, and then repeat Step 7. 8. Transfer the supernatant to a new nuclease-free 15 ml centrifuge tube containing one volume of 100% isopropanol. Do not transfer the protein pellet. 9. Gently mix the solution by inverting the tube 40-50 times. 10
Mag-Bind Blood DNA Maximum Yield Protocol (7ml-10ml)
实验试剂 1. Absolute ethanol (96%-100%) 实验设备 1. Nuclease-free 50 ml centrifuge tube 2. Water bath, incubator or heating block preset at 65°C 3. Magnetic separation device for 50 ml tubes
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