Pierce(R) Iodination Tubes

Preparation of total RNA from whole blood collected in PAXgene tubes

Short PAXgene Protocol:spin in step 6 increased to 20 min; no Dnase treatment; optional wash after step 12 REQUIRED1.Centrifuge the PAXgene Blood RNA Tube for 10 min at 30005000 x g using a swing-out rotor.Note: Ensure that the blood sample

R 语言的高颜值的配图法则 | 论文写作

如下：What？这完全没有办法聚类好么，我还怎么看差异变化？把这样的热图给老板，别说文章凉了，能不能走出老板办公室都是问题。图片来源：影视截图现在的高分论文一个个华丽丽的配图，动辄 R 语言、Python…听起来就高级，笔者当年凭着中学的记忆笨笨地操作 Excel，真是苦不堪言！老板说了，要是再做不出来满意的图，就滚去刷一个月锥形瓶。于是就有小伙伴问了，没时间系统地学习编程，那种「套代码公式就能搞定 R 语言绘图，懂中文就能会的教程」，能不能给我来一份啊？答案是肯定的了，下面就跟随笔者，从作图小白一步步做个初级大触

ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES

Need 1.5-2 x 107 cells from a 2 day culture. 1. Cells are harvested as normal, washed x 1 in PBS then taken up at conc. of 1.2 x 10 7 cells/ml in cold PBS. 2. Take x2 Biorad curvettes A. 0.9 ml (107 cells) B. 0.167 ml cells (2 x 106 cells