O-GlcNAc Western Blot

Antibodies and Activity Measurements for the Detection of O-GlcNAc Transferase and Assay of its Substrate, UDP-GlcNAc

Since the discovery of O -GlcNAc modification (O -GlcNAcylation) 20 years ago, much attention has been given to OGT (O -GlcNAc transferase), the unique enzyme responsible for the nuclear and cytosolic O -GlcNAcylation processes. This review

升级Western blot可以同时检测大量蛋白

来自芝加哥大学癌症生物研究所，本-梅癌症研究中心等处的研究人员发明了一种能同时检测大量蛋白的新方法，彻底改变目前我们进行癌症和其它疾病蛋白表达水平检测的技术面貌，这种新方法效果与传统的Western Blotting一样好，但是时间可以大大缩短，比传统Western Blotting快48倍。这一研究成果公布在《Nature Methods》杂志上。这种称为“micro-western arrays”（微印迹分析）的方法能将蛋白检测常用实验：Western blot的特异识别能力，和DNA芯片

Western Blot Detection of Adrenergic Receptors

(often referred to as Western blotting), which is used to identify specific antigens recognized by antibodies (1 ). Membrane-bound or purified adrenergic receptors (ARs) are solubilized with sodium dodecyl sulfate (SDS) and reduced with dithiothreitol (DTT