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Amine-Functionalized eFluor® 700NC 250 uL
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文献和实验RNASafer® Protocol for Total RNA Isolation
appropriate E.Z.N.A.® Total RNA Kit. Use RNA Miniprep Kit (R6634) for less than 40mg tissue, Total RNA Midiprep kit (R6664) for 200mg tissue, RNA Maxiprep Kit(R6693) for 1 gram tissue. 3) For RNA isolation using RNA miniprep kit, add 700ul TRK lysis
E.Z.N.A.® Protocol for Bacteria
-500ul DEPC-treated water (supplied with kit). Centrifuge column for 10 min at 4,000-5,000 x g. If the expected RNA yield > 500 ug the a second elution may be required. Elution with two 250 ul aliquots is no more efficient than with one 500 ul aliquot
E.Z.N.A.® Yeast Plasmid Mini Spin Protocol
good yields. Incubate at 30°C for at least 30 min. 4. Pellet spheroblasts by centrifuging at 4,000 x g for 5 min at room temperature. Discard the supernatant completely. 5. Resuspend the spheroblasts pellet with 250 ul Buffer YP I/RNase
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