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- 抗体英文名：
Anti-Mouse/Rat TNF alpha Purified 50 ug
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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
than this you can put two slides face to face (acting as each other's coverslip) with 30m l of hyb solution. 50m l of Hybridisation solution Solution A 6mls de-ionised formamide 2mls 20 x SSC 2mls H2 O 2g dextran sulphate
Production and Characterization of Recombinant Human and Murine TNF
takes about 2 wk. Human and murine TNF can be purified from crude extracts with high yields (>50 mg from 1 L of fermentation) by hydrophobic-interaction chromatography, ion-exchange chromatography, and gel-filtration chromatography. The purity
Intracellular Cytokine Staining Protocol
immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinMP6-XT22TNF-aNEW!mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinTN3-19.12Annotations: mouse PEC=mouse thioglycolate
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