Anti-CD257 (BAFF, BLyS) Purifi

RNA Purification Protocol for 10-20mg of Animal Tissue

buffer. Cellular proteins and genomic DNA are removed by precipitation with PNP Buffer while RNA will remain in solution. RNA is further purified by isopropanol precipitation. 实验试剂   1. Isopropanol 2. 70% ethanol 实验

用 CD3/CD28 做好 T 细胞激活与扩增

分钟收集细胞； 5. 将上一步离心得到的细胞沉淀，重悬至扩增培养基中，并进行计数。 1.3 细胞刺激（次日） 扩增培养基成分：RPMI1640 + 10% 血清 + 5ug/ml anti-mouse CD28（克隆号：37.51） + 双抗 1. 将细胞浓度调整至 1-2×10^6/ml（本次实验为 2×10^6/ml，该浓度偏大）； 2. 从 4°C 冰箱取出前一天包被的板子，弃掉包被液，无菌PBS洗涤3遍； 3. 每孔加入 500μl 细胞悬液，放置于 37°C 含 5% CO2 细胞

RUVKUN Antibody Staining Protocol revised 10/29/90

from O2 pressure. Wash once with BO3 buffer and once for 15 min. or more with Antibody Buffer B: 9. Store worms in Antibody Buffer A: 10. Incubate a small aliquot of worms in 10ug/ml RNAse A, 1 hour, 37 C. Stain RNAsed and unRNAsed aliquots (small