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Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP(R) Rabbit mAb
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
. Biochem. J. 408:297‐315. Blenis, J., Kuo, C.J., and Erikson, R.L. 1987. Identification of a ribosomal protein S6 kinase regulated by transformation and growth‐promoting stimuli
at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
3.1 and 3.6 ). This will provide a “specific activity” measurement relative to total protein in the cell, and is themost accurate measure of total GSK3 activity, or2. Immunoblot using antibodies to phospho-Ser21 (GSK3a)or phospho-Ser9 (GSK3b) (see Sections 3.1 –
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