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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
ANTI-WASL/N-WASP RABBIT PAB
- 供应商：
北京智杰方远科技
- CAS号：
******
- 保存条件：
详见说明
- 规格：
****
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
by adding 1 mL PBS, 3' each. Add 0.3 mL rabbit anti-GFP (1:200 v/v, plus 1% goat serum in PBS) and incubate 1 hr. at room temp. Wash 3x in PBS. Post-fix in 1 mL 4% paraformaldehyde x15', room temp. Wash 3x in PBS. Denature DNA with 1 mL
ELISA (Enzyme-Linked ImmunoadSorbent Assay)
for at least 1hr at 37°C. 5) Remove BSA by flicking plate. 6) Wash 3x with 1x PBS containing 0.01% tween 7) Add anti-serum (diluted as appropiate, 1/100 etc. with 1x PBS) and incubate for 2hrs @ 37°C. N.B.: Use positive and negative controls. 8) Wash 4x
then serially diluted in 100 µl assay buffer on the plate). Incubate @ RT for 1 hr while shaking. Meanwhile let TMB solution equilibrate to RT. Wash plate 3x and add 100 µl/well rabbit polyclonal anti-p27 antibody diluted 1:1000 in assay buffer
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