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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
MS CD45 HRZN V500 MAB 0.05MG 30-F11
- 保存条件:
-20度
- 规格:
**
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文献和实验on ice. Add Triton X-100 and Tween-20 to the homogenate at final concentrations of 4% (v/v) and 1 mg/ml, respectively, and homogenize briefly to mix. Stir the homogenate on ice for 30 min. 2. Centrifuge at 25,400 x g for 30 min (Sorvall GSA rotor, 12
Dual- and Triple-Color Fluorospot
labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
antibody (mAb) conjugated with peroxidase (anti-DNA-POD) was used. This anti-DNA-POD mAb binds to single- and double-stranded, low molecular weight DNA fragments (mono- and oligonucleosomes), showing the internucleosomal degradation of genomic DNA occuring
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