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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
HU IGG HRP MAB 1.0ML G18-145
- 保存条件:
-20度
- 规格:
**
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文献和实验the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody preparation (serum, tissue culture supernatant, or ascites).Apply Binding buffer again ~ 200 ml.Apply Elution buffer ~ 100 ml.Collect 3 ml
. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody preparation (serum, tissue culture supernatant, or ascites). Apply Binding buffer again ~ 200 ml. Apply Elution buffer ~ 100 ml. Collect 3 ml fractions in 5 ml
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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