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- 抗体英文名:
MS MAC-3 FITC MAB 0.5MG M3/84
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-20度
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文献和实验Dual- and Triple-Color Fluorospot
labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml
Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
Actinomycin D (AD) stock solution (1 mg/mL) 7-aminoactinomycin D (7-AAD) stock solution (1 mg/mL) Antibody staining solution Human cells of interest, fresh or cultured (or immortalized human cell lines) Monoclonal antibodies (mAb
H2O 1.84 g 柠檬酸 0.51 g DDW 100 ml 6. 显色液(现配现用):底物Buffer 10 ml OPD 2 mg 30% H2O2 2 ml 7. 终止液 2 Mol/L H2SO4 8. 重组人TFAR19, HRP标记的羊抗人IgG9. ELISA板, Tip, 移液器,ELISA Reader(OD490 nm),洗板机 操作步骤 1. 用包被Buffer稀释的重组人TFAR19(1 mg/ml)包被ELISA板, 100 ml/well, 37
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