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- 抗体英文名：
eEF1A1
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10ug
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文献和实验Signature-tagged mutagenesis (STM) was originally developed by David Holden while studying the filamentous fungus Aspergillus fumigatus . In attempts to define virulence determinants for this pathogenic fungus, candidate factors were selected
HIS-tagged protein purification
John Mundy,Institute of Molecular Biology,Copenhagen,Denmark 1 liter cell prep 1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about 1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze at 70℃ 2)resuspend
Affinity Immobilization of Tagged Enzymes
for enzyme immobilization. This work describes experimental strategies for affinity immobilization of tagged enzymes onto activated supports. Protocols for attaching histidine (His) and sugar-tagged enzymes onto common supports such as commercial aminated
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