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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
ZO1 tight junction
- 规格：
10ug
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文献和实验Claudin-4: Functional Studies Beyond the Tight Junction
Claudin-4 is an unusual member of the claudin family; in addition to its role in epithelial tight junction barrier function, it is a receptor for the Clostridium perfringens enterotoxin. We have also found that claudin-4 is regulated
Immunoprecipitation is one of the most commonly used techniques to study protein-protein interaction in vivo. There are three major steps involved in an immunoprecipitation procedure: 1) lysis of the cells to make the antigen
HIS-tagged protein purification
John Mundy,Institute of Molecular Biology,Copenhagen,Denmark 1 liter cell prep 1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about 1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze at 70℃ 2)resuspend
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