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- 文献和实验
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- 抗体英文名:
F4/80
- 保存条件:
4°C/-20°C
- 规格:
50ug
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
Immunohistochemistry using Anti-Ganglioside Antibodies
Acetone Mouse MAb to ganglioside Mouse MAb for control experiment FITC-conjugated goat F(ab')2 fragment to mouse IgM (µ chain-specific) FITC-conjugated goat F(ab')2 fragment to mouse IgG
b) Determine and record A280 and A492. c) Calculate protein concentration: protein (mg/mL) = (A280- (A492 x 0.35))/1.4 d) Calculate moles of protein: = protein(mg/mL)/1.5x10^5 e) Calculate moles of FITC: = A492/0.69x10^5 f) Determine F
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